VEGFR2/CD309 anticorps (Biotin)

N° du produit ABIN1589717

L’anticorps Souris Monoclonal anti-VEGFR2/CD309 a été validé pour WB, IHC, ELISA et FACS. Il convient pour détecter VEGFR2/CD309 dans des échantillons de Humain.

Aperçu rapide pour VEGFR2/CD309 anticorps (Biotin) (ABIN1589717)

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VEGFR2/CD309 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3(4H3

Détail du produit anti-VEGFR2/CD309 anticorps (Biotin)

Fonction: VEGFR-2/KDR antibody

Specificité: Recombinant human soluble extracellular KDR (D7)

Réactivité croisée (Details): The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Attributs du produit: Chromosomal location: 4q11-q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Purification: Mouse IgG1 antibody (clone 3 (4H3)) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogène: Recombinant human soluble extracellular KDR (D7)

Isotype: IgG1

Information d'application

Indications d'application: FACS: Use at 2-10 μg/mL, Western Blot: Use at 2-5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Centrifuge vial prior to opening.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Date de péremption: 24 months

Détails sur VEGFR2/CD309

Antigène: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Autre désignation: VEGFR-2/KDR

Sujet: Vascular endothelial growth factor receptor-2 , KDR, FLK1, CD309, VEGF receptor 2, VEGFR2, kinase insert domain protein receptor,VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

ID gène: 3791

NCBI Accession: NM_002253, NP_002244

UniProt: P35968

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling